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anti rabbit mouse igg secondary antibodies  (Vector Laboratories)


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    Vector Laboratories anti rabbit mouse igg secondary antibodies
    Anti Rabbit Mouse Igg Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 19583 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+rabbit+igg+secondary+antibody/Biotinylated+Goat+Anti-Rabbit+IgG+Antibody/pmc13062622-169-6-14
    Average 96 stars, based on 19583 article reviews
    anti rabbit mouse igg secondary antibodies - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Vaccination reduces shedding of salmonid alphavirus subtype 3, but bacterial co-infection influences the effect
    Article Snippet: .. Following primary antibody incubation, sections were incubated with a biotinylated horse anti-mouse/rabbit IgG secondary antibody (Vector Laboratories, BA-1400) for 20 minutes, followed by incubation with VECTASTAIN ABC–AP reagent (Vector Laboratories, AK-5001) for 30 minutes at room temperature. .. Immunoreactivity was visualized using the ImmPACT Vector Red AP substrate kit (Vector Laboratories, SK-5105) for 20–30 minutes.

    Article Title: Conventional ambient temperature housing impacts central oxytocin levels in mice
    Article Snippet: Sections were then rinsed for 5 min three times in KPBS and then incubated in 1% goat serum (Thermo Fisher Scientific) + KPBS +0.4% Triton-X for 30 min at room temperature. .. The tissue was then incubated in biotinylated goat anti-mouse/rabbit IgG secondary antibody (1:600 dilution in KPBS + 0.4% Triton-X, Vector Laboratories) for 60 min, and then rinsed for 5 min five times in KPBS. .. Next, tissue was incubated in avidin–biotin peroxidase complex (Vector Labs; Vectastain Elite ABC Universal kit; 45 μL A, 45 μL B per 10 mL KPBS + 0.4% Triton-X) for 60 min, and then rinsed in KPBS for 5 min three times.

    Article Title: Full-length ATP7B reconstituted through protein trans -splicing corrects Wilson disease in mice
    Article Snippet: For immunohistochemistry, 5-μm-thick sections were rehydrated and permeabilized in PBS/0.2-Triton (Sigma) for 20 min. Antigen unmasking was performed in 0.01 M citrate buffer in a microwave oven. .. Next, sections underwent blocking of endogenous peroxidase activity in methanol/1.5% H 2 O 2 (Sigma) for 30 min and incubated with blocking solution (3% BSA [Sigma], 5% donkey serum [Millipore], 1.5% horse serum [Vector Laboratories] 20 mM MgCl 2 , 0.3% Triton [Sigma] in PBS] for 1 h. Sections were incubated with primary antibody (rabbit anti-ATP7B, Thermo Fisher Scientific, Cat#PA-102826, dilution: 1/100) overnight at 4°C and then with universal biotinylated horse anti-mouse/rabbit IgG secondary antibody (Vector Laboratories) for 1 h. Biotin/avidin-HRP signal amplification was achieved using the ABC Elite Kit (Vector Laboratories) according to manufacturer’s instructions. .. 3,3′-diaminobenzidine (Vector Laboratories) was used as the peroxidase substrate.

    Article Title: Impact of Cesarean Delivery on Reward Behavior and Tyrosine Hydroxylase in the Nucleus Accumbens of Adult Prairie Vole Offspring
    Article Snippet: Sections were then rinsed for 5 min three consecutive times in KPBS and then incubated in 1% horse serum (Thermo Fisher Scientific) + KPBS + 0.4% Triton-X (KPBS TX ) for 30 min at room temperature. .. The tissue was then incubated in horse anti-mouse/rabbit IgG secondary antibody (Vector Labs; 1:600 dilution in KPBS TX ) for 60 min at room temperature, then rinsed for 5 min five consecutive times in KPBS. .. Next, tissue was incubated in avidin–biotin peroxidase complex (Vector Labs; Vectastain Elite ABC Universal kit; 45 μL A, 45 μL B per 10 mL KPBS TX ) for 60 min at room temperature and then rinsed in KPBS for 5 min three consecutive times.

    Article Title: Full-length ATP7B reconstituted through protein trans -splicing corrects Wilson disease in mice.
    Article Snippet: For immunohistochemistry, 5-mm-thick sections were rehydrated and permeabilized in PBS/0.2-Triton (Sigma) for 20 min. Antigen unmasking was performed in 0.01 M citrate buffer in a microwave oven. .. Next, sections underwent blocking of endogenous peroxidase activity in methanol/1.5% H2O2 (Sigma) for 30 min and incubated with blocking solution (3% BSA [Sigma], 5% donkey serum [Millipore], 1.5% horse serum [Vector Laboratories] 20 mM MgCl2, 0.3% Triton [Sigma] in PBS] for 1 h. Sections were incubated with primary antibody (rabbit anti-ATP7B, Thermo Fisher Scientific, Cat#PA102826, dilution: 1/100) overnight at 4 C and then with universal biotinylated horse anti-mouse/rabbit IgG secondary antibody (Vector Laboratories) for 1 h. Biotin/avidin-HRP signal amplification was achieved using the ABC Elite Kit (Vector Laboratories) according to manufacturer’s instructions. .. 3,30-diaminobenzidine (Vector Laboratories) was used as the peroxidase substrate.

    Blocking Assay:

    Article Title: Full-length ATP7B reconstituted through protein trans -splicing corrects Wilson disease in mice
    Article Snippet: For immunohistochemistry, 5-μm-thick sections were rehydrated and permeabilized in PBS/0.2-Triton (Sigma) for 20 min. Antigen unmasking was performed in 0.01 M citrate buffer in a microwave oven. .. Next, sections underwent blocking of endogenous peroxidase activity in methanol/1.5% H 2 O 2 (Sigma) for 30 min and incubated with blocking solution (3% BSA [Sigma], 5% donkey serum [Millipore], 1.5% horse serum [Vector Laboratories] 20 mM MgCl 2 , 0.3% Triton [Sigma] in PBS] for 1 h. Sections were incubated with primary antibody (rabbit anti-ATP7B, Thermo Fisher Scientific, Cat#PA-102826, dilution: 1/100) overnight at 4°C and then with universal biotinylated horse anti-mouse/rabbit IgG secondary antibody (Vector Laboratories) for 1 h. Biotin/avidin-HRP signal amplification was achieved using the ABC Elite Kit (Vector Laboratories) according to manufacturer’s instructions. .. 3,3′-diaminobenzidine (Vector Laboratories) was used as the peroxidase substrate.

    Article Title: Full-length ATP7B reconstituted through protein trans -splicing corrects Wilson disease in mice.
    Article Snippet: For immunohistochemistry, 5-mm-thick sections were rehydrated and permeabilized in PBS/0.2-Triton (Sigma) for 20 min. Antigen unmasking was performed in 0.01 M citrate buffer in a microwave oven. .. Next, sections underwent blocking of endogenous peroxidase activity in methanol/1.5% H2O2 (Sigma) for 30 min and incubated with blocking solution (3% BSA [Sigma], 5% donkey serum [Millipore], 1.5% horse serum [Vector Laboratories] 20 mM MgCl2, 0.3% Triton [Sigma] in PBS] for 1 h. Sections were incubated with primary antibody (rabbit anti-ATP7B, Thermo Fisher Scientific, Cat#PA102826, dilution: 1/100) overnight at 4 C and then with universal biotinylated horse anti-mouse/rabbit IgG secondary antibody (Vector Laboratories) for 1 h. Biotin/avidin-HRP signal amplification was achieved using the ABC Elite Kit (Vector Laboratories) according to manufacturer’s instructions. .. 3,30-diaminobenzidine (Vector Laboratories) was used as the peroxidase substrate.

    Activity Assay:

    Article Title: Full-length ATP7B reconstituted through protein trans -splicing corrects Wilson disease in mice
    Article Snippet: For immunohistochemistry, 5-μm-thick sections were rehydrated and permeabilized in PBS/0.2-Triton (Sigma) for 20 min. Antigen unmasking was performed in 0.01 M citrate buffer in a microwave oven. .. Next, sections underwent blocking of endogenous peroxidase activity in methanol/1.5% H 2 O 2 (Sigma) for 30 min and incubated with blocking solution (3% BSA [Sigma], 5% donkey serum [Millipore], 1.5% horse serum [Vector Laboratories] 20 mM MgCl 2 , 0.3% Triton [Sigma] in PBS] for 1 h. Sections were incubated with primary antibody (rabbit anti-ATP7B, Thermo Fisher Scientific, Cat#PA-102826, dilution: 1/100) overnight at 4°C and then with universal biotinylated horse anti-mouse/rabbit IgG secondary antibody (Vector Laboratories) for 1 h. Biotin/avidin-HRP signal amplification was achieved using the ABC Elite Kit (Vector Laboratories) according to manufacturer’s instructions. .. 3,3′-diaminobenzidine (Vector Laboratories) was used as the peroxidase substrate.

    Article Title: Full-length ATP7B reconstituted through protein trans -splicing corrects Wilson disease in mice.
    Article Snippet: For immunohistochemistry, 5-mm-thick sections were rehydrated and permeabilized in PBS/0.2-Triton (Sigma) for 20 min. Antigen unmasking was performed in 0.01 M citrate buffer in a microwave oven. .. Next, sections underwent blocking of endogenous peroxidase activity in methanol/1.5% H2O2 (Sigma) for 30 min and incubated with blocking solution (3% BSA [Sigma], 5% donkey serum [Millipore], 1.5% horse serum [Vector Laboratories] 20 mM MgCl2, 0.3% Triton [Sigma] in PBS] for 1 h. Sections were incubated with primary antibody (rabbit anti-ATP7B, Thermo Fisher Scientific, Cat#PA102826, dilution: 1/100) overnight at 4 C and then with universal biotinylated horse anti-mouse/rabbit IgG secondary antibody (Vector Laboratories) for 1 h. Biotin/avidin-HRP signal amplification was achieved using the ABC Elite Kit (Vector Laboratories) according to manufacturer’s instructions. .. 3,30-diaminobenzidine (Vector Laboratories) was used as the peroxidase substrate.

    Amplification:

    Article Title: Full-length ATP7B reconstituted through protein trans -splicing corrects Wilson disease in mice
    Article Snippet: For immunohistochemistry, 5-μm-thick sections were rehydrated and permeabilized in PBS/0.2-Triton (Sigma) for 20 min. Antigen unmasking was performed in 0.01 M citrate buffer in a microwave oven. .. Next, sections underwent blocking of endogenous peroxidase activity in methanol/1.5% H 2 O 2 (Sigma) for 30 min and incubated with blocking solution (3% BSA [Sigma], 5% donkey serum [Millipore], 1.5% horse serum [Vector Laboratories] 20 mM MgCl 2 , 0.3% Triton [Sigma] in PBS] for 1 h. Sections were incubated with primary antibody (rabbit anti-ATP7B, Thermo Fisher Scientific, Cat#PA-102826, dilution: 1/100) overnight at 4°C and then with universal biotinylated horse anti-mouse/rabbit IgG secondary antibody (Vector Laboratories) for 1 h. Biotin/avidin-HRP signal amplification was achieved using the ABC Elite Kit (Vector Laboratories) according to manufacturer’s instructions. .. 3,3′-diaminobenzidine (Vector Laboratories) was used as the peroxidase substrate.

    Article Title: Full-length ATP7B reconstituted through protein trans -splicing corrects Wilson disease in mice.
    Article Snippet: For immunohistochemistry, 5-mm-thick sections were rehydrated and permeabilized in PBS/0.2-Triton (Sigma) for 20 min. Antigen unmasking was performed in 0.01 M citrate buffer in a microwave oven. .. Next, sections underwent blocking of endogenous peroxidase activity in methanol/1.5% H2O2 (Sigma) for 30 min and incubated with blocking solution (3% BSA [Sigma], 5% donkey serum [Millipore], 1.5% horse serum [Vector Laboratories] 20 mM MgCl2, 0.3% Triton [Sigma] in PBS] for 1 h. Sections were incubated with primary antibody (rabbit anti-ATP7B, Thermo Fisher Scientific, Cat#PA102826, dilution: 1/100) overnight at 4 C and then with universal biotinylated horse anti-mouse/rabbit IgG secondary antibody (Vector Laboratories) for 1 h. Biotin/avidin-HRP signal amplification was achieved using the ABC Elite Kit (Vector Laboratories) according to manufacturer’s instructions. .. 3,30-diaminobenzidine (Vector Laboratories) was used as the peroxidase substrate.



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